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Yeungnam Univ J Med > Volume 10(2); 1993 > Article
Yeungnam University Journal of Medicine 1993;10(2):360-368.
DOI: https://doi.org/10.12701/yujm.1993.10.2.360    Published online December 31, 1993.
Isolation of GTP binding from bovine brain.
Jung Hye Kim
Abstract
GTP binding protein (G-protein) associated with membrane and involved in signal transduction was isolated from bovine brain, and molecular weight of G protein was observed. As the results, cell membranes were homogenized from bovine brain tissues and proteins of membrane were gained using 1% cholate, and progressed the chromatography. The purification process was performed by step, DEAE-Sephacel, Ulttrogel AcA 34 and heptylamine-Sepharose column chromatography. The chromatographic fractions were confirmed by GTP binding assay and SDS-polyacrylamide gel electrophoresis. Molecular weight of Goa was revealed 39,000 dalton and GR 36,000 dalton. One more step of heptylamine-Sepharose was enforced to purify the GTP binding protein. Finally I gained the GTP binding protein isolated subtype of Goalpha and Gbeta.


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